›› 2012, Vol. 24 ›› Issue (2): 91-95.doi: 10.3969/j.issn.1004-616x.2012.02.003

• 论著 • Previous Articles     Next Articles

Changes of TGF-β signal pathway in the process of 16HBE malignant transformation induced by glycidyl methacrylate

WANG An-na，DONG Lin，WANG Quan-kai，HU Jie，XU Jian-ning   

  1. 1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050; 2. Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
  • Received:2011-08-30 Revised:2011-12-05 Online:2012-03-30 Published:2012-03-30
  • Contact: XU Jian-ning

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Abstract: OBJECTIVE: To analyze the changes of transforming growth factor beta (TGF-β) signal pathway among different stages of malignant transformation of human bronchial epithelial cells (16HBE) induced by glycidyl methacrylate (GMA) and to discuss the molecular mechanisms in the process. METHODS：Cells were harvested at different time points，the 10th generation (protophase) and the 30th (anaphase) generation cells transformed by GMA and the control group in the same generation. Then the changes of TGF-β signal pathway were analyzed by "Human Genome Expression Chip" in the process. RESULTS：TGF-β signaling pathway in cell of the 30th generation showed significant differences between the transformed and control groups，and there were 11 differentially expressed genes. But there were no significant differences in cells of the 10th generation. CONCLUSION ：TGF-β signal pathway possibly plays an important role in the process of malignant transformation of 16HBE induced by GMA.

Key words: glycidyl methacrylate, human bronchial epithelial cells, gene expression pathways